Plasmid
Part:BBa_K3579023:Design
Designed by: Amandine Maire Group: iGEM20_Paris_Bettencourt (2020-10-27)
EpiFlex_pPBEF1_mCherry_AB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 3509
Illegal SpeI site found at 1682
Illegal PstI site found at 1812 - 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 13
Illegal BsaI site found at 103
Illegal BsaI site found at 175
Illegal BsaI site found at 994
Illegal BsaI site found at 8007
Illegal BsaI.rc site found at 65
Illegal BsaI.rc site found at 137
Illegal BsaI.rc site found at 956
Illegal BsaI.rc site found at 1105
Illegal BsaI.rc site found at 7154
Illegal SapI site found at 4265
Illegal SapI site found at 5041
Design Notes
BsaI and BbsI restriction sites have been removed
Source
pRMC2 (Plasmid #68940)