Plasmid

Part:BBa_K3579023:Design

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF1_mCherry_AB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3509
    Illegal SpeI site found at 1682
    Illegal PstI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI site found at 103
    Illegal BsaI site found at 175
    Illegal BsaI site found at 994
    Illegal BsaI site found at 8007
    Illegal BsaI.rc site found at 65
    Illegal BsaI.rc site found at 137
    Illegal BsaI.rc site found at 956
    Illegal BsaI.rc site found at 1105
    Illegal BsaI.rc site found at 7154
    Illegal SapI site found at 4265
    Illegal SapI site found at 5041


Design Notes

BsaI and BbsI restriction sites have been removed



Source

pRMC2 (Plasmid #68940)


References